Although I already worked around this problem by finding the x-intercept with the linear regression equation I calculated w/gnuplot, I'm still curious to know how pgfplots can do it without me manually computing it.
\documentclass[12pt]{article}
\usepackage[T1]{fontenc}
\usepackage[utf8]{inputenc}
\usepackage{lmodern,tikz,pgfplots,pgfplotstable}
\begin{document}
\begin{center}
\begin{tikzpicture}
\begin{axis}[axis on top=false, axis x line=middle, axis y line=middle,xlabel=$\mathrm{\frac{1}{[S]}}$,ylabel=$\mathrm{\frac{1}{\textit{V}_0}}$,
xmin=-10000,xmax=10000,ymin=-0.01,ymax=0.035]
\addplot table [y={create col/linear regression={}}]
{
X Y
10000 0.030
5000 0.02
2000 0.014
1000 0.012
500 0.0110
200 0.0104
100 0.0102
50 0.010
20 0.01
10 0.01
5 0.01
-4999.85 0
-9995.35 -0.01
};
\xdef\slope{\pgfplotstableregressiona}
\xdef\slope{\pgfplotstableregressionb}
\end{axis}
\end{tikzpicture}
\end{center}
\end{document}
