The minus sign and the fraction line aren't perfectly aligned, probably due to the presence of brackets. So I've found a way to make them be, using \parbox
\documentclass[12pt]{article}
\usepackage{amsmath}
\begin{document}
\begin{equation}
\dfrac{N_{i}}{N_{0}} = \exp \bigg( \parbox[h][2cm][c]{4mm}{$-$} \dfrac{{E_i - E_0}}{KT} \bigg)
\end{equation}
\end{document}
Is there a more elegant way to achieve this?
\parbox[h] parbox
does not have anh
option, also\bigg(
should be\biggl(
, there is no need to use\frac
here display mode fractions are automatic in an equation If you want to adjust the height it would be more natural to use\raisebox